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Exogenous Carbohydrate Effects on Thermoadaptation and Thermostress in Ogataea parapolymorpha Under Different Carbon Sourcesстатья Исследовательская статья
- Авторы: Atroshenko Denis, Roslova Alexandra, Yakobson Anastasia, Markova Diana, Golovina Diana, Tishkov Vladimir
- Журнал: Fermentation
- Том: 12
- Номер: 4
- Год издания: 2026
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 199
- DOI: 10.3390/fermentation12040199
- Аннотация: Thermotolerant methylotrophic yeast Ogataea parapolymorpha is a promising host for high-temperature bioprocesses, yet the effects of carbon source and exogenous carbohydrates on their heat response remain poorly understood. We investigated how growth on glucose, glycerol, or methanol, short-term thermoadaptation (45 °C, 2 h), and supplementation with trehalose, sucrose, maltose, or xylose affect thermotolerance (55 °C, 30 min) and intracellular trehalose content. Thermoadaptation increased survival on all carbon sources and was accompanied by substantial trehalose accumulation in glucose- and glycerol-grown cells, but only minor trehalose accumulation in methanol-grown cells. Carbohydrate supplementation improved survival only in methanol-grown cultures. Under these conditions, trehalose, sucrose, and maltose increased intracellular trehalose levels, whereas xylose enhanced survival without a comparable increase in trehalose. These results show that the heat-stress response of O. parapolymorpha is strongly carbon source-dependent and that the protective effects of carbohydrate supplementation in methanol-grown cells cannot be explained by trehalose accumulation alone.
- Добавил в систему: Атрошенко Денис Леонидович