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PEG-Chitosan and Glycol-Chitosan for Improvement of Biopharmaceutical Propertiesof Recombinant L-Asparaginase from Erwinia carotovoraстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 апреля 2015 г.
- Авторы: Sukhoverkov K.V., Kudryashova E.V.
- Журнал: Biochemistry (Moscow)
- Том: 80
- Номер: 1
- Год издания: 2015
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 113
- Последняя страница: 119
- DOI: 10.1134/S0006297915010137
- Аннотация: Conjugation with the new branched copolymers, PEGchitosan and glycolchitosan, is suggested to improve the therapeutic properties of Lasparaginase from Erwinia carotovora (EwA). The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 36 compared to the native enzyme. This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer. The thermostability of EwA conjugates was also considerably improved. ChitoPEGylation, similarly to PEGylation, can be expected to improve pharmacokinetic properties and to reduce immunogenicity of this medically relevant enzyme. It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEGchitosan copolymer composition as well as composition of copoly mer–enzyme conjugates. The proposed analytic method is “reagentfree” and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
- Добавил в систему: Кудряшова Елена Вадимовна