Effects of gamma irradiation on collagen damage and remodelingстатья
Статья опубликована в высокорейтинговом журнале
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 5 мая 2015 г.
Аннотация:Purpose: To evaluate the dose-time dependences of structural
changes occurring in collagen within 24 hours to three months
after gamma-irradiation at doses from 2–40 Gy in vivo.
Materials and methods: Rat’s tail tendon was chosen as in vivo
model, with its highly ordered collagen structure allowing the
changes to be interpreted unambiguously. Macromolecular level
(I) was investigated by differential scanning calorimetry (DSC);
fibers and bundles level (II) by laser scanning microscopy (LSM),
and bulk tissue microstructural level (III) by cross-polarization
optical coherence tomography (CP-OCT).
Results: For (I), the formation of molecular cross-links and breaks
appeared to be a principal mechanism of collagen remodeling,
with the cross-links number dependent on radiation dose.
Changes on level (II) involved primary, secondary and tertiary
bundles splitting in a day and a week after irradiation. Bulk collagen
microstructure (III) demonstrated early widening of the
interference fringes on CP-OCT images observed to occur in the
tendon as result of this splitting. At all three levels, the observed
collagen changes demonstrated complete remodeling within ∼ a
month following irradiation.
Conclusion: The time course and dose dependencies of the
observed collagen changes at different levels of its hierarchy further
contribute to elucidating the role of connective tissue in the
radiotherapy process.