Macro, Micro, and Nano-Biosensors: Potential Applications and Possible Limitations, Editors: Rai, M., Reshetilov, A., Plekhanova, Y., Ingle, A.P. (Eds.). Chapter 9. Enhanced Sensitivity Rapid Tests for the Detection of Sepsis Marker Procalcitonin

Serebrennikova, K.V.; Samsonova, J.V.; Osipov, A.P.

Авторы: Serebrennikova Kseniya V., Samsonova Jeanne V., Osipov Alexander P.
Год издания: 2021
Издательство: Springer International Publishing AG
Местоположение издательства: Cham, Switzerland
Объём: 175 страниц
ISBN: 978-3-030-55489-7
DOI: 10.1007/978-3-030-55490-3_9
Аннотация: Lateral flow immunoassay is a widely used method due to its simplicity and rapidity. However, insufficient sensitivity in the case of a biomarker detection within the clinical ranges when necessary is a limiting factor to implement this method for point-of-care testing and other purposes. This chapter is focused on the approaches to improve the sensitivity (limit of detection) of rapid lateral flow immunoassay on the example of sepsis biomarker procalcitonin. The existing enhancement approaches can be divided into two groups: detectable labels combined with sensitive registration devices and additional signal amplification procedures. The effect of label shape and size (spherical and hierarchical gold nanoparticles) on the detection limit of procalcitonin and additional procedures to enhance the analytical signal of the assay are discussed. The potential of combining lateral flow immunoassay with highly sensitive surface-enhanced Raman spectroscopy is considered. In terms of a simple test for semi-quantitative visual marker detection in several clinically relevant concentration ranges, the one-step gradient lateral flow immunoassay is proposed.
Добавил в систему: Осипов Александр Павлович

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Macro, Micro, and Nano-Biosensors: Potential Applications and Possible Limitations, Editors: Rai, M., Reshetilov, A., Plekhanova, Y., Ingle, A.P. (Eds.). Chapter 9. Enhanced Sensitivity Rapid Tests for the Detection of Sepsis Marker Procalcitoninкнига

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