Место издания:Mary Ann Liebert Inc United States United States
Первая страница:136
Последняя страница:136
Аннотация:A stem cell niche is a structural and functional microenvi-
ronment supporting and regulating a stem cell pool. Mesench-
ymal stromal cells (MSC), as a part of stem cell niche, maintain
many types of tissue-specific resident stem cells particularly
through the production of multiple bioactive factors and thus
coordinating processes of tissue repair and regeneration. In
spermatogonial stem cell (SSC) niche, cells functionally similar
to MSC were also found, but their role remained uncovered.
However, MSC can be crucial for SSC niche regulation as they
produce paracrine factors affecting principal components of SSC
niche by modulating cell proliferation, survival and differentia-
tion. We studied the contribution of MSC secretome to SSC
niche regulation within the development of a new MSC
secretome-based drug for the treatment of male infertility. Using
a model of rat bilateral abdominal cryptorchidism for reversible
SSC niche injury we observed that MSC secretome components
effectively restored spermatogenesis. By immunohistochemical
analysis of testis sections we observed regeneration of the whole
testicle, not only distinct seminiferous tubules. A direct corre-
lation between number of tubules containing many proliferating
cells and produced spermatozoa number, and an inverse corre-
lation between the interstitial cell proliferation level and pro-
duced spermatozoa number were found. We also conducted
in vitro evaluation of survival and proliferation of specific SSC
niche cells to confirm in vivo data. Our findings suggest MSC
could regulate SSC niche by paracrine signals and support
spermatogenesis.